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SVEN_5003 is a Major Developmental Regulator in Streptomyces venezuelae

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Abstract

Many regulatory genes that affect cellular development in Streptomyces, such as the canonical bld genes, have already been identified. However, in this study, we identified sven_5003 in Streptomyces venezuelae as a major new developmental regulatory gene, the deletion of which leads to a bald phenotype, typical of bld mutants, under multiple growth conditions. Our data indicated that disruption of sven_5003 also has a differential impact on the production of the two antibiotics jadomycin and chloramphenicol. Enhanced production of jadomycin but reduced production of chloramphenicol were detected in our sven_5003 mutant strain (S. venezuelae D5003). RNA-Seq analysis indicated that SVEN_5003 impacts expression of hundreds of genes, including genes involved in development, primary and secondary metabolism, and genes of unknown function, a finding confirmed by real-time PCR analysis. Transcriptional analysis indicated that sven_5003 is an auto-regulatory gene, repressing its own expression. Despite the evidence indicating that SVEN_5003 is a regulatory factor, a putative DNA-binding domain was not predicted from its primary amino acid sequence, implying an unknown regulatory mechanism by SVEN_5003. Our findings revealed that SVEN_5003 is a pleiotropic regulator with a critical role in morphological development in S. venezuelae.

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Funding

This work was supported by grants from the National Natural Science Foundation of China (NSF32270072 to XP) and from the Qingdao Natural Science Foundation (23-2-1-27-zyyd-jch to YZ).

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Authors and Affiliations

  1. The State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237, China

    Yan** Zhu, Ting Lu, Hanlei Zhang & **-Zhu-Aff1">Yan** Zhu

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  2. Ting Lu
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  3. Hanlei Zhang
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  4. Meng Liu
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  5. **uhua Pang
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Contributions

XP conceived and supervised the study. YZ and TL performed the experiments and collected data. XP, YZ, HZ, and ML analyzed the data. XP wrote the manuscript. All authors read and approved the manuscript.

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Correspondence to **uhua Pang.

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Zhu, Y., Lu, T., Zhang, H. et al. SVEN_5003 is a Major Developmental Regulator in Streptomyces venezuelae. Curr Microbiol 81, 166 (2024). https://doi.org/10.1007/s00284-024-03688-8

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