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Open AccessDesign of an automated robotic microinjection system for batch injection of zebrafish embryos and larvae
This paper presents the design of a vision-based automated robotic microinjection system for batch injection of both zebrafish embryos and larvae. A novel visual recognition algorithm based on an automatic thr...
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Article
Open AccessPublisher Correction: Genetic evidence for differential functions of figla and nobox in zebrafish ovarian differentiation and folliculogenesis
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Open AccessGenetic evidence for differential functions of figla and nobox in zebrafish ovarian differentiation and folliculogenesis
FIGLA and NOBOX are important oocyte-specific transcription factors. Both figla-/- and nobox-/- mutants showed all-male phenotype in zebrafish due to increased dominance of the male-promoting pathway. The early d...
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Article
Open AccessCrowded environments tune the fold-switching in metamorphic proteins
Metamorphic proteins such as circadian clock protein KaiB and human chemokine XCL1 play vital roles in regulating biological processes, including gene expression, circadian clock and innate immune responses, a...
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Article
Open AccessNicotinamide promotes cardiomyocyte derivation and survival through kinase inhibition in human pluripotent stem cells
Nicotinamide, the amide form of Vitamin B3, is a common nutrient supplement that plays important role in human fetal development. Nicotinamide has been widely used in clinical treatments, including the treatme...
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Separation of Oocyte and Follicle Layer for Gene Expression Analysis in Zebrafish
Zebrafish ovarian follicles are mainly composed of the oocyte and a thin layer of follicle cells. Recent studies have demonstrated extensive cell-cell interactions between the oocyte and surrounding follicle l...
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Article
Open AccessPresenilin 1 deficiency suppresses autophagy in human neural stem cells through reducing γ-secretase-independent ERK/CREB signaling
Autophagy impairment is commonly implicated in the pathological characteristic of Alzheimer’s disease (AD). Presenilin 1 (PS1) expression in human brain gradually decreases with age and its mutations account f...
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Roles of Nitric Oxide Synthase Isoforms in Neurogenesis
Nitric oxide (NO), a free radical gas, acts as a neurotransmitter or neuromodulator in the central nervous system (CNS). It has been widely explored as a mediator of neuroinflammation, neuronal damages, and ne...
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Article
Open AccessDiscovery of a ROCK inhibitor, FPND, which prevents cerebral hemorrhage through maintaining vascular integrity by interference with VE-cadherin
Hemorrhagic stroke occurs when a weakened vessel ruptures and bleeds into the surrounding brain, leading to high rates of death and disability worldwide. A series of complex pathophysiological cascades contrib...
